Detailed information of TA system TA099517

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3317657..3318028
• Replicon: chromosome
• Accession: NZ_CP027577
• Organism: Escherichia coli strain 2013C-4225

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: C6997_RS18060
• Protein ID: WP_001443846.1
• Coordinates: 3317879..3318028 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3317657..3317835 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6997_RS18035	3313612..3314985	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
C6997_RS18040	3315114..3316049	-	936	WP_001157401.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C6997_RS18045	3316101..3317336	-	1236	WP_000040851.1	site-specific integrase	-
C6997_RS18050	3317338..3317553	-	216	WP_000079604.1	excisionase XisR	-
C6997_RS18055	3317653..3317841	-	189	WP_001302840.1	DUF1187 family protein	-
-	3317657..3317835	+	179	NuclAT_0	-	Antitoxin
-	3317657..3317835	+	179	NuclAT_0	-	Antitoxin
-	3317657..3317835	+	179	NuclAT_0	-	Antitoxin
-	3317657..3317835	+	179	NuclAT_0	-	Antitoxin
C6997_RS18060	3317879..3318028	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C6997_RS18065	3318084..3318893	-	810	WP_000166313.1	recombination protein RecT	-
C6997_RS18070	3318886..3321486	-	2601	WP_000105150.1	exodeoxyribonuclease VIII	-
C6997_RS18075	3321588..3321863	-	276	WP_000632297.1	protein RacC	-
C6997_RS18080	3321938..3322108	-	171	WP_001352098.1	conserved protein, Rac prophage	-
C6997_RS18085	3322108..3322329	-	222	WP_000560223.1	killing protein KilR	-
C6997_RS18090	3322750..3322902	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	3316101..3380055	63954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T99517 WP_001443846.1 NZ_CP027577:c3318028-3317879 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T99517 NZ_CP027577:c3318028-3317879 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT99517 NZ_CP027577:3317657-3317835 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin