Detailed information of TA system TA099503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 751093..751695
• Replicon: chromosome
• Accession: NZ_CP027577
• Organism: Escherichia coli strain 2013C-4225

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6997_RS03885
• Protein ID: WP_000897305.1
• Coordinates: 751093..751404 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6997_RS03890
• Protein ID: WP_000356397.1
• Coordinates: 751405..751695 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6997_RS03855	746123..746908	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
C6997_RS03860	747007..747606	+	600	WP_001315111.1	glucose-1-phosphatase	-
C6997_RS03865	747600..748472	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6997_RS03870	748469..748906	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6997_RS03875	748951..749892	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6997_RS03880	749956..750864	-	909	WP_001162704.1	alpha/beta hydrolase	-
C6997_RS03885	751093..751404	+	312	WP_000897305.1	hypothetical protein	Toxin
C6997_RS03890	751405..751695	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6997_RS03900	752300..752518	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6997_RS03905	752737..752979	+	243	WP_001086390.1	hypothetical protein	-
C6997_RS03910	753310..754239	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6997_RS03915	754236..754871	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
C6997_RS03920	754868..755770	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99503 WP_000897305.1 NZ_CP027577:751093-751404 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99503 NZ_CP027577:751093-751404 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99503 WP_000356397.1 NZ_CP027577:751405-751695 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99503 NZ_CP027577:751405-751695 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7