Detailed information of TA system TA099500

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73993..74262
• Replicon: plasmid unnamed3
• Accession: NZ_CP027576
• Organism: Escherichia coli strain 2013C-4081

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: A2F94_RS30440
• Protein ID: WP_001312861.1
• Coordinates: 74104..74262 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	73993..74058 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2F94_RS30400	69217..69441	-	225	WP_106905013.1	hypothetical protein	-
A2F94_RS30415	69786..70313	+	528	WP_000290792.1	single-stranded DNA-binding protein	-
A2F94_RS30420	70369..70602	+	234	WP_000006004.1	DUF905 domain-containing protein	-
A2F94_RS30425	70661..72619	+	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
A2F94_RS30430	72674..73108	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
A2F94_RS30435	73105..73824	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
A2F94_RS30955	73836..74024	-	189	WP_001299721.1	hypothetical protein	-
-	73836..74060	+	225	NuclAT_0	-	-
-	73836..74060	+	225	NuclAT_0	-	-
-	73836..74060	+	225	NuclAT_0	-	-
-	73836..74060	+	225	NuclAT_0	-	-
-	73993..74058	+	66	-	-	Antitoxin
A2F94_RS30440	74104..74262	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
A2F94_RS31170	74616..74840	-	225	WP_001427866.1	hypothetical protein	-
A2F94_RS30460	75184..75471	+	288	WP_000107535.1	hypothetical protein	-
A2F94_RS30465	75592..76413	+	822	WP_001234469.1	DUF945 domain-containing protein	-
A2F94_RS30470	76710..77219	-	510	Protein_83	transglycosylase SLT domain-containing protein	-
A2F94_RS30475	77633..78016	+	384	WP_001151524.1	relaxosome protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..78427	78427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T99500 WP_001312861.1 NZ_CP027576:74104-74262 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T99500 NZ_CP027576:74104-74262 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT99500 NZ_CP027576:73993-74058 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin