Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 5191758..5192260 Replicon chromosome
Accession NZ_CP027573
Organism Escherichia coli strain 2013C-4081

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A2F94_RS27455 Protein ID WP_000767829.1
Coordinates 5192006..5192260 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A2F94_RS27450 Protein ID WP_001259255.1
Coordinates 5191758..5192009 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2F94_RS27420 5186929..5187996 - 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
A2F94_RS27425 5187993..5189072 - 1080 WP_000102292.1 histidinol-phosphate transaminase -
A2F94_RS27430 5189069..5190373 - 1305 WP_001345258.1 histidinol dehydrogenase -
A2F94_RS27435 5190379..5191278 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
A2F94_RS27445 5191424..5191474 - 51 WP_001364200.1 his operon leader peptide -
A2F94_RS27450 5191758..5192009 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A2F94_RS27455 5192006..5192260 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A2F94_RS27460 5192343..5193167 + 825 WP_000754755.1 SDR family oxidoreductase -
A2F94_RS27465 5193213..5194142 + 930 WP_000803331.1 LysR family transcriptional regulator -
A2F94_RS27470 5194357..5194419 + 63 WP_010723108.1 membrane protein YoeI -
A2F94_RS27475 5194409..5195767 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A2F94_RS27480 5195946..5197004 + 1059 WP_000492340.1 transport protein YeeE -
A2F94_RS27485 5197018..5197245 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99496 WP_000767829.1 NZ_CP027573:5192006-5192260 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99496 NZ_CP027573:5192006-5192260 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99496 WP_001259255.1 NZ_CP027573:5191758-5192009 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99496 NZ_CP027573:5191758-5192009 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References