Detailed information of TA system TA099494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4384265..4384992
• Replicon: chromosome
• Accession: NZ_CP027573
• Organism: Escherichia coli strain 2013C-4081

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: A2F94_RS23225
• Protein ID: WP_000547559.1
• Coordinates: 4384265..4384576 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A2F94_RS23230
• Protein ID: WP_000126294.1
• Coordinates: 4384573..4384992 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2F94_RS23195	4379407..4381116	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
A2F94_RS23200	4381126..4381668	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
A2F94_RS23205	4381668..4382435	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
A2F94_RS23210	4382432..4382842	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
A2F94_RS23215	4382835..4383305	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
A2F94_RS23220	4383348..4384091	+	744	WP_001369451.1	hypothetical protein	-
A2F94_RS23225	4384265..4384576	+	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
A2F94_RS23230	4384573..4384992	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
A2F94_RS23235	4385106..4386530	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
A2F94_RS23240	4386539..4387996	-	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
A2F94_RS23245	4388256..4389266	+	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
A2F94_RS23250	4389415..4389942	+	528	WP_001078781.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T99494 WP_000547559.1 NZ_CP027573:4384265-4384576 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T99494 NZ_CP027573:4384265-4384576 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT99494 WP_000126294.1 NZ_CP027573:4384573-4384992 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT99494 NZ_CP027573:4384573-4384992 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163