Detailed information of TA system TA099490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3004926..3005528
• Replicon: chromosome
• Accession: NZ_CP027573
• Organism: Escherichia coli strain 2013C-4081

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A2F94_RS16080
• Protein ID: WP_000897305.1
• Coordinates: 3005217..3005528 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A2F94_RS16075
• Protein ID: WP_000356397.1
• Coordinates: 3004926..3005216 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2F94_RS16045	3000851..3001753	+	903	WP_000331369.1	formate dehydrogenase O subunit beta	-
A2F94_RS16050	3001750..3002385	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
A2F94_RS16055	3002382..3003311	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
A2F94_RS16060	3003642..3003884	-	243	WP_001086388.1	hypothetical protein	-
A2F94_RS16065	3004103..3004321	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
A2F94_RS16075	3004926..3005216	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
A2F94_RS16080	3005217..3005528	-	312	WP_000897305.1	hypothetical protein	Toxin
A2F94_RS16085	3005757..3006665	+	909	WP_001162704.1	alpha/beta hydrolase	-
A2F94_RS16090	3006729..3007670	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A2F94_RS16095	3007715..3008152	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
A2F94_RS16100	3008149..3009021	-	873	WP_000920762.1	virulence factor BrkB family protein	-
A2F94_RS16105	3009015..3009614	-	600	WP_001315111.1	glucose-1-phosphatase	-
A2F94_RS16110	3009713..3010498	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99490 WP_000897305.1 NZ_CP027573:c3005528-3005217 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99490 NZ_CP027573:c3005528-3005217 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99490 WP_000356397.1 NZ_CP027573:c3005216-3004926 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99490 NZ_CP027573:c3005216-3004926 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7