Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5443654..5444234 Replicon chromosome
Accession NZ_CP027561
Organism Pseudomonas fluorescens strain G7

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag C6Y56_RS24785 Protein ID WP_085747693.1
Coordinates 5443654..5443932 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C6Y56_RS24790 Protein ID WP_045121630.1
Coordinates 5443947..5444234 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6Y56_RS24765 5439236..5439403 - 168 WP_007950992.1 DUF2474 domain-containing protein -
C6Y56_RS24770 5439461..5440468 - 1008 WP_169432006.1 cytochrome d ubiquinol oxidase subunit II -
C6Y56_RS24775 5440472..5441908 - 1437 WP_169432007.1 cytochrome ubiquinol oxidase subunit I -
C6Y56_RS24780 5442309..5443547 + 1239 WP_169432008.1 MFS transporter -
C6Y56_RS24785 5443654..5443932 + 279 WP_085747693.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6Y56_RS24790 5443947..5444234 + 288 WP_045121630.1 HigA family addiction module antidote protein Antitoxin
C6Y56_RS24795 5444240..5444791 - 552 WP_169432009.1 DJ-1/PfpI family protein -
C6Y56_RS24800 5444858..5445307 - 450 WP_169432010.1 DUF4879 domain-containing protein -
C6Y56_RS24805 5445528..5446823 + 1296 WP_169432011.1 NCS2 family permease -
C6Y56_RS24810 5446820..5447899 + 1080 WP_169432012.1 tRNA (uridine(54)-C5)-methyltransferase TrmA -
C6Y56_RS24815 5447955..5448401 - 447 WP_169432013.1 hypothetical protein -
C6Y56_RS24820 5448398..5448826 - 429 WP_039771768.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-75)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10673.22 Da        Isoelectric Point: 8.9401

>T99467 WP_085747693.1 NZ_CP027561:5443654-5443932 [Pseudomonas fluorescens]
MIKSFQHKGLRGFYETGSTRGIRADHAKRLSRMLQFMDRAAAPGDLDLPGWRLHPLKGELCEYWSLSVSGNWRVIFRFAG
SDIELIDYLDYH

Download         Length: 279 bp

>T99467 NZ_CP027561:5443654-5443932 [Pseudomonas fluorescens]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGCGGCTTCTATGAAACCGGTTCGACTCGCGGGATTCGTGCCGATCACGC
TAAACGGCTGTCGCGCATGCTGCAGTTCATGGATCGCGCAGCGGCTCCCGGGGATCTGGACCTTCCCGGTTGGCGTCTGC
ATCCACTGAAAGGGGAGCTTTGCGAGTACTGGTCACTCAGTGTGTCCGGGAACTGGCGGGTTATCTTTCGATTTGCAGGT
TCGGATATCGAACTGATCGACTATCTGGATTACCACTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10542.17 Da        Isoelectric Point: 6.9219

>AT99467 WP_045121630.1 NZ_CP027561:5443947-5444234 [Pseudomonas fluorescens]
MPMHNPPHPGETLLMDVLPELGISVTELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARMWLGVQTDYDLWQA
EHREQPAIQPIAAHA

Download         Length: 288 bp

>AT99467 NZ_CP027561:5443947-5444234 [Pseudomonas fluorescens]
ATGCCTATGCACAACCCGCCACACCCCGGTGAAACACTGCTGATGGACGTATTGCCGGAACTTGGGATCAGCGTGACCGA
ATTGGCTCGGCATCTTGGATTCGCTCGTCCTCACCTTTCCCGTGTTTTGCACGGGCATGCGCCGATCAGCCCGGATCTGG
CCGTGCGACTGGAACGCGCGGGGATTGGCAAGGCTCGCATGTGGCTAGGCGTTCAAACCGATTACGACCTTTGGCAAGCC
GAGCACCGTGAGCAGCCAGCCATTCAACCAATCGCTGCTCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References