Detailed information of TA system TA099462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 332659..333293
• Replicon: plasmid unnamed1
• Accession: NZ_CP027558
• Organism: Rhodococcus rhodochrous strain BX2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: C6Y44_RS26360
• Protein ID: WP_192378936.1
• Coordinates: 333012..333293 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6Y44_RS26355
• Protein ID: WP_192378935.1
• Coordinates: 332659..332952 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6Y44_RS26330	328892..330148	-	1257	WP_192378933.1	type II toxin-antitoxin system HipA family toxin	-
C6Y44_RS26335	330145..330432	-	288	WP_016691920.1	helix-turn-helix domain-containing protein	-
C6Y44_RS26340	330674..330754	+	81	Protein_268	TetR/AcrR family transcriptional regulator	-
C6Y44_RS26345	331128..331721	+	594	WP_192378934.1	hypothetical protein	-
C6Y44_RS26350	331760..332236	+	477	Protein_270	glutathione-dependent formaldehyde dehydrogenase	-
C6Y44_RS26355	332659..332952	-	294	WP_192378935.1	HigA family addiction module antidote protein	Antitoxin
C6Y44_RS26360	333012..333293	-	282	WP_192378936.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6Y44_RS26365	333392..333793	-	402	WP_192378937.1	YceI family protein	-
C6Y44_RS26370	334131..334463	+	333	WP_192378938.1	N-acetyltransferase	-
C6Y44_RS26375	334460..334939	+	480	WP_192378939.1	carboxymuconolactone decarboxylase family protein	-
C6Y44_RS26380	335126..335905	-	780	WP_192378940.1	ABC transporter ATP-binding protein	-
C6Y44_RS26385	335902..337017	-	1116	WP_192378941.1	iron ABC transporter permease	-
C6Y44_RS26390	337014..337994	-	981	WP_192379108.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..423264	423264

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10850.29 Da
• Isoelectric Point: 10.1821

>T99462 WP_192378936.1 NZ_CP027558:c333293-333012 [Rhodococcus rhodochrous]
VIESFADKGTERVFHRQFSKKLPQDIQRIARRRLLLVDAATDINDLRAPPGNRLEKLAGNRAGQYSVRINDQYRICFTWR
TTAAYNVEITDYH

Nucleotide

• Length: 282 bp

>T99462 NZ_CP027558:c333293-333012 [Rhodococcus rhodochrous]
GTGATCGAATCGTTCGCCGACAAAGGGACCGAGCGAGTCTTTCACCGGCAGTTCTCCAAGAAACTCCCACAGGACATTCA
GCGGATCGCACGCCGGCGACTGCTGCTGGTCGACGCGGCTACCGATATCAACGATCTGCGGGCACCTCCGGGCAACCGCC
TGGAGAAGCTCGCCGGGAACCGCGCGGGCCAGTACAGCGTTCGGATCAACGACCAGTACCGCATCTGCTTCACCTGGCGC
ACCACAGCCGCATACAACGTAGAAATCACCGACTACCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10688.21 Da
• Isoelectric Point: 6.7242

>AT99462 WP_192378935.1 NZ_CP027558:c332952-332659 [Rhodococcus rhodochrous]
MTADLSPIHPGEILREDFLEPLGITQNRLATTIGVPPRRINEIVHGKRSITADTALRLAKAFGNSAQFWLNIQDRYELDI
AADKLGDTLAGIHRLSA

Nucleotide

• Length: 294 bp

>AT99462 NZ_CP027558:c332952-332659 [Rhodococcus rhodochrous]
ATGACTGCAGACCTGAGCCCCATTCACCCCGGCGAGATCCTCCGTGAGGACTTCCTCGAACCGCTCGGCATCACCCAGAA
CCGCCTTGCCACCACCATCGGTGTGCCCCCACGACGGATCAACGAGATCGTGCACGGAAAACGCTCGATCACCGCAGACA
CCGCACTGCGGCTGGCCAAGGCATTCGGCAACAGCGCACAGTTCTGGCTCAACATCCAGGACCGCTACGAACTCGATATC
GCAGCAGACAAGTTGGGCGACACGCTTGCCGGTATCCATCGACTCTCGGCATAA