Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2108944..2109479 Replicon chromosome
Accession NZ_CP027555
Organism Escherichia coli strain 2013C-3513

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag C6991_RS12385 Protein ID WP_032183258.1
Coordinates 2109192..2109479 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6991_RS12380 Protein ID WP_000729704.1
Coordinates 2108944..2109204 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6991_RS12355 2103981..2105120 + 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -
C6991_RS12360 2105104..2107197 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
C6991_RS12365 2107302..2107580 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6991_RS12370 2107573..2107929 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6991_RS12375 2107986..2108758 - 773 Protein_2153 C40 family peptidase -
C6991_RS12380 2108944..2109204 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6991_RS12385 2109192..2109479 + 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6991_RS12390 2109635..2110375 + 741 WP_001225679.1 murein L,D-transpeptidase -
C6991_RS12395 2110346..2111113 - 768 WP_000333380.1 class II glutamine amidotransferase -
C6991_RS12400 2111319..2111897 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T99449 WP_032183258.1 NZ_CP027555:2109192-2109479 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T99449 NZ_CP027555:2109192-2109479 [Escherichia coli]
ATTAGGAATTTAAATCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAA
GGATATGAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACC
CGCTGCAAGGTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAA
CTTTTACGATTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT99449 WP_000729704.1 NZ_CP027555:2108944-2109204 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT99449 NZ_CP027555:2108944-2109204 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References