Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1192426..1192928 Replicon chromosome
Accession NZ_CP027555
Organism Escherichia coli strain 2013C-3513

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6991_RS07500 Protein ID WP_000767829.1
Coordinates 1192426..1192680 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6991_RS07505 Protein ID WP_001259255.1
Coordinates 1192677..1192928 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6991_RS07470 1187441..1187668 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6991_RS07475 1187682..1188740 - 1059 WP_000492324.1 transport protein YeeE -
C6991_RS07480 1188919..1190277 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6991_RS07485 1190267..1190329 - 63 WP_010723108.1 membrane protein YoeI -
C6991_RS07490 1190544..1191473 - 930 WP_000803351.1 LysR family transcriptional regulator -
C6991_RS07495 1191519..1192343 - 825 WP_000754758.1 SDR family oxidoreductase -
C6991_RS07500 1192426..1192680 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6991_RS07505 1192677..1192928 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6991_RS07510 1193211..1193261 + 51 WP_001364200.1 his operon leader peptide -
C6991_RS07520 1193407..1194306 + 900 WP_000131756.1 ATP phosphoribosyltransferase -
C6991_RS07525 1194312..1195616 + 1305 WP_001442151.1 histidinol dehydrogenase -
C6991_RS07530 1195613..1196683 + 1071 WP_000108960.1 histidinol-phosphate transaminase -
C6991_RS07535 1196683..1197750 + 1068 WP_000080110.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99446 WP_000767829.1 NZ_CP027555:c1192680-1192426 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99446 NZ_CP027555:c1192680-1192426 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99446 WP_001259255.1 NZ_CP027555:c1192928-1192677 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99446 NZ_CP027555:c1192928-1192677 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References