Detailed information of TA system TA099426

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34902..35171
• Replicon: plasmid unnamed1
• Accession: NZ_CP027554
• Organism: Escherichia coli strain 2013C-3513

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C6991_RS00310
• Protein ID: WP_001312861.1
• Coordinates: 35013..35171 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	34902..34967 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6991_RS00265	29962..30222	+	261	WP_071600428.1	hypothetical protein	-
C6991_RS00280	30695..31222	+	528	WP_000290816.1	single-stranded DNA-binding protein	-
C6991_RS00285	31278..31511	+	234	WP_058653442.1	DUF905 domain-containing protein	-
C6991_RS00290	31570..33528	+	1959	WP_058653444.1	ParB/RepB/Spo0J family partition protein	-
C6991_RS00295	33583..34017	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
C6991_RS00300	34014..34733	+	720	WP_033807739.1	plasmid SOS inhibition protein A	-
-	34745..34969	+	225	NuclAT_0	-	-
-	34745..34969	+	225	NuclAT_0	-	-
-	34745..34969	+	225	NuclAT_0	-	-
-	34745..34969	+	225	NuclAT_0	-	-
-	34902..34967	+	66	-	-	Antitoxin
C6991_RS00310	35013..35171	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C6991_RS31460	35409..35786	-	378	Protein_53	hypothetical protein	-
C6991_RS00325	36086..36382	+	297	WP_001272251.1	hypothetical protein	-
C6991_RS00330	36493..37314	+	822	WP_021503380.1	DUF945 domain-containing protein	-
C6991_RS00335	37610..38257	-	648	WP_072208664.1	transglycosylase SLT domain-containing protein	-
C6991_RS00340	38543..38926	+	384	WP_001151564.1	relaxosome protein TraM	-
C6991_RS00345	39120..39806	+	687	WP_000332487.1	PAS domain-containing protein	-
C6991_RS00350	39901..40128	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / tet(B)	-	1..70129	70129

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T99426 WP_001312861.1 NZ_CP027554:35013-35171 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T99426 NZ_CP027554:35013-35171 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT99426 NZ_CP027554:34902-34967 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin