Detailed information of TA system TA099421

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 6565..6834
• Replicon: plasmid unnamed
• Accession: NZ_CP027553
• Organism: Escherichia coli strain 2015C-4498

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C6953_RS29230
• Protein ID: WP_001312861.1
• Coordinates: 6676..6834 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	6565..6630 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6953_RS30225	1837..2148	+	312	WP_199851519.1	hypothetical protein	-
C6953_RS29200	2378..2905	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
C6953_RS29205	2961..3194	+	234	WP_000006004.1	DUF905 domain-containing protein	-
C6953_RS29210	3253..5211	+	1959	WP_001145473.1	ParB/RepB/Spo0J family partition protein	-
C6953_RS29215	5266..5700	+	435	WP_044804925.1	conjugation system SOS inhibitor PsiB	-
C6953_RS29220	5697..6460	+	764	Protein_7	plasmid SOS inhibition protein A	-
-	6429..6632	+	204	NuclAT_0	-	-
-	6429..6632	+	204	NuclAT_0	-	-
-	6429..6632	+	204	NuclAT_0	-	-
-	6429..6632	+	204	NuclAT_0	-	-
-	6565..6630	+	66	-	-	Antitoxin
C6953_RS29230	6676..6834	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C6953_RS30230	7524..7730	+	207	WP_000547971.1	hypothetical protein	-
C6953_RS29250	7755..8042	+	288	WP_000107535.1	hypothetical protein	-
C6953_RS30235	8162..8245	+	84	Protein_11	DUF945 domain-containing protein	-
C6953_RS29260	8430..9617	-	1188	WP_106884016.1	IS91 family transposase	-
C6953_RS29265	9617..9982	-	366	WP_000091308.1	hypothetical protein	-
C6953_RS29275	10440..11420	-	981	WP_000019402.1	IS5-like element IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyB / hlyD / espP	1..67055	67055

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T99421 WP_001312861.1 NZ_CP027553:6676-6834 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T99421 NZ_CP027553:6676-6834 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT99421 NZ_CP027553:6565-6630 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin