Detailed information of TA system TA099416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4867090..4867817
• Replicon: chromosome
• Accession: NZ_CP027552
• Organism: Escherichia coli strain 2015C-4498

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: C6953_RS26250
• Protein ID: WP_000547559.1
• Coordinates: 4867506..4867817 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6953_RS26245
• Protein ID: WP_000126294.1
• Coordinates: 4867090..4867509 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6953_RS26225	4862140..4862667	-	528	WP_001078781.1	electron transport protein HydN	-
C6953_RS26230	4862816..4863826	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
C6953_RS26235	4864086..4865543	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C6953_RS26240	4865552..4866976	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
C6953_RS26245	4867090..4867509	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C6953_RS26250	4867506..4867817	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6953_RS26255	4867991..4868734	-	744	WP_001341829.1	hypothetical protein	-
C6953_RS26260	4868777..4869247	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C6953_RS26265	4869240..4869650	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C6953_RS26270	4869647..4870414	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
C6953_RS26275	4870414..4870956	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
C6953_RS26280	4870966..4872675	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T99416 WP_000547559.1 NZ_CP027552:c4867817-4867506 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T99416 NZ_CP027552:c4867817-4867506 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT99416 WP_000126294.1 NZ_CP027552:c4867509-4867090 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT99416 NZ_CP027552:c4867509-4867090 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163