Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3992898..3993400 Replicon chromosome
Accession NZ_CP027552
Organism Escherichia coli strain 2015C-4498

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6953_RS21580 Protein ID WP_000767829.1
Coordinates 3992898..3993152 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6953_RS21585 Protein ID WP_001259255.1
Coordinates 3993149..3993400 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6953_RS21550 3987913..3988140 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6953_RS21555 3988154..3989212 - 1059 WP_044805324.1 transport protein YeeE -
C6953_RS21560 3989391..3990749 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6953_RS21565 3990739..3990801 - 63 WP_010723108.1 membrane protein YoeI -
C6953_RS21570 3991016..3991945 - 930 WP_000803337.1 LysR family transcriptional regulator -
C6953_RS21575 3991991..3992815 - 825 WP_000754758.1 SDR family oxidoreductase -
C6953_RS21580 3992898..3993152 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6953_RS21585 3993149..3993400 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6953_RS21590 3993683..3993733 + 51 WP_001368802.1 his operon leader peptide -
C6953_RS21600 3993879..3994778 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6953_RS21605 3994784..3996088 + 1305 WP_106884165.1 histidinol dehydrogenase -
C6953_RS21610 3996085..3997155 + 1071 WP_106884166.1 histidinol-phosphate transaminase -
C6953_RS21615 3997155..3998222 + 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99414 WP_000767829.1 NZ_CP027552:c3993152-3992898 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99414 NZ_CP027552:c3993152-3992898 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99414 WP_001259255.1 NZ_CP027552:c3993400-3993149 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99414 NZ_CP027552:c3993400-3993149 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References