Detailed information of TA system TA099394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 485311..485913
• Replicon: chromosome
• Accession: NZ_CP027552
• Organism: Escherichia coli strain 2015C-4498

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6953_RS02530
• Protein ID: WP_000897305.1
• Coordinates: 485602..485913 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6953_RS02525
• Protein ID: WP_100039178.1
• Coordinates: 485311..485601 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6953_RS02490	481237..482139	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C6953_RS02495	482136..482771	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
C6953_RS02500	482768..483697	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6953_RS02510	484028..484270	-	243	WP_001086388.1	hypothetical protein	-
C6953_RS02515	484489..484707	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6953_RS02525	485311..485601	-	291	WP_100039178.1	helix-turn-helix domain-containing protein	Antitoxin
C6953_RS02530	485602..485913	-	312	WP_000897305.1	hypothetical protein	Toxin
C6953_RS02535	486142..487050	+	909	WP_001162704.1	alpha/beta hydrolase	-
C6953_RS02540	487114..488055	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6953_RS02545	488100..488537	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6953_RS02550	488534..489406	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C6953_RS02555	489400..489999	-	600	WP_001315111.1	glucose-1-phosphatase	-
C6953_RS02560	490098..490883	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99394 WP_000897305.1 NZ_CP027552:c485913-485602 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99394 NZ_CP027552:c485913-485602 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCTCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10793.38 Da
• Isoelectric Point: 10.1864

>AT99394 WP_100039178.1 NZ_CP027552:c485601-485311 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRCASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99394 NZ_CP027552:c485601-485311 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCTGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7