Detailed information of TA system TA099390

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 160565..160991
• Replicon: plasmid unnamed
• Accession: NZ_CP027551
• Organism: Escherichia coli strain 2015C-4136CT1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: C6950_RS26435
• Protein ID: WP_062860169.1
• Coordinates: 160565..160723 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	160767..160991 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6950_RS26390	155629..155868	-	240	WP_042357785.1	conjugal transfer relaxosome protein TraY	-
C6950_RS26395	155976..156623	-	648	WP_074464494.1	transcriptional regulator TraJ family protein	-
C6950_RS26400	156815..157198	-	384	WP_001151530.1	relaxosome protein TraM	-
C6950_RS26405	157612..158121	+	510	Protein_190	transglycosylase SLT domain-containing protein	-
C6950_RS26410	158417..159238	-	822	WP_074453754.1	DUF945 domain-containing protein	-
C6950_RS26965	159357..159539	-	183	WP_170979925.1	hypothetical protein	-
C6950_RS26970	159668..159874	-	207	WP_062897188.1	hypothetical protein	-
C6950_RS26435	160565..160723	-	159	WP_062860169.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	160767..160991	-	225	NuclAT_0	-	Antitoxin
-	160767..160991	-	225	NuclAT_0	-	Antitoxin
-	160767..160991	-	225	NuclAT_0	-	Antitoxin
-	160767..160991	-	225	NuclAT_0	-	Antitoxin
C6950_RS26750	160803..160991	+	189	WP_001299721.1	hypothetical protein	-
C6950_RS26440	161003..161722	-	720	WP_024221815.1	plasmid SOS inhibition protein A	-
C6950_RS26445	161719..162153	-	435	WP_062860170.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	bfpK / bfpJ / bfpI / bfpH / bfpP / bfpF / bfpE / bfpD / bfpB / bfpG / bfpA	1..162810	162810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6096.34 Da
• Isoelectric Point: 9.2584

>T99390 WP_062860169.1 NZ_CP027551:c160723-160565 [Escherichia coli]
MKLPRSSLIWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T99390 NZ_CP027551:c160723-160565 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTATCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT99390 NZ_CP027551:c160991-160767 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin