Detailed information of TA system TA099382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3639087..3639689
• Replicon: chromosome
• Accession: NZ_CP027550
• Organism: Escherichia coli strain 2015C-4136CT1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: C6950_RS19115
• Protein ID: WP_000897302.1
• Coordinates: 3639087..3639398 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6950_RS19120
• Protein ID: WP_000356397.1
• Coordinates: 3639399..3639689 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6950_RS19090	3635001..3635600	+	600	WP_001314324.1	glucose-1-phosphatase	-
C6950_RS19095	3635594..3636466	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6950_RS19100	3636463..3636900	+	438	WP_021560109.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6950_RS19105	3636945..3637886	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6950_RS19110	3637950..3638858	-	909	WP_001357065.1	alpha/beta hydrolase	-
C6950_RS19115	3639087..3639398	+	312	WP_000897302.1	hypothetical protein	Toxin
C6950_RS19120	3639399..3639689	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6950_RS19130	3640048..3640326	+	279	WP_001296612.1	hypothetical protein	-
C6950_RS19140	3640722..3640940	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
C6950_RS19145	3641164..3642093	-	930	WP_000027710.1	formate dehydrogenase accessory protein FdhE	-
C6950_RS19150	3642090..3642725	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6950_RS19155	3642722..3643624	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T99382 WP_000897302.1 NZ_CP027550:3639087-3639398 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99382 NZ_CP027550:3639087-3639398 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99382 WP_000356397.1 NZ_CP027550:3639399-3639689 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99382 NZ_CP027550:3639399-3639689 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416