Detailed information of TA system TA099381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 3203221..3204539
• Replicon: chromosome
• Accession: NZ_CP027550
• Organism: Escherichia coli strain 2015C-4136CT1

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: C6950_RS16915
• Protein ID: WP_021560080.1
• Coordinates: 3203221..3204228 (-)
• Length: 336 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2A7M399
• Locus tag: C6950_RS16920
• Protein ID: WP_001490601.1
• Coordinates: 3204228..3204539 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6950_RS16875	3198914..3199105	+	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
C6950_RS16880	3199102..3200781	+	1680	WP_021560079.1	cellulose biosynthesis protein BcsG	-
C6950_RS16885	3200867..3200974	-	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
-	3201023..3201088	+	66	NuclAT_23	-	-
-	3201023..3201088	+	66	NuclAT_23	-	-
-	3201023..3201088	+	66	NuclAT_23	-	-
-	3201023..3201088	+	66	NuclAT_23	-	-
-	3201023..3201088	+	66	NuclAT_25	-	-
-	3201023..3201088	+	66	NuclAT_25	-	-
-	3201023..3201088	+	66	NuclAT_25	-	-
-	3201023..3201088	+	66	NuclAT_25	-	-
C6950_RS26670	3201350..3201457	-	108	WP_000170747.1	type I toxin-antitoxin system toxin Ldr family protein	-
-	3201506..3201571	+	66	NuclAT_24	-	-
-	3201506..3201571	+	66	NuclAT_24	-	-
-	3201506..3201571	+	66	NuclAT_24	-	-
-	3201506..3201571	+	66	NuclAT_24	-	-
-	3201506..3201571	+	66	NuclAT_26	-	-
-	3201506..3201571	+	66	NuclAT_26	-	-
-	3201506..3201571	+	66	NuclAT_26	-	-
-	3201506..3201571	+	66	NuclAT_26	-	-
C6950_RS16910	3201933..3203204	+	1272	WP_001312176.1	amino acid permease	-
C6950_RS16915	3203221..3204228	-	1008	WP_021560080.1	HipA domain-containing protein	Toxin
C6950_RS16920	3204228..3204539	-	312	WP_001490601.1	HipA N-terminal domain-containing protein	Antitoxin
C6950_RS16925	3204524..3204847	-	324	WP_021560081.1	helix-turn-helix transcriptional regulator	-
C6950_RS16930	3205072..3206085	-	1014	WP_001357946.1	dipeptide ABC transporter ATP-binding subunit DppF	-
C6950_RS16935	3206082..3207065	-	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	-
C6950_RS16940	3207076..3207978	-	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
C6950_RS16945	3207988..3209007	-	1020	WP_074453901.1	dipeptide ABC transporter permease DppB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-302)

Antitoxin

• HipA, N-terminal subdomain 1 (6-98)

Sequences

Toxin

Protein

• Length: 336 a.a.
• Molecular weight: 38395.65 Da
• Isoelectric Point: 5.4135

>T99381 WP_021560080.1 NZ_CP027550:c3204228-3203221 [Escherichia coli]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDYKGLPVHQEQLDGAMQITD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPTLAPVYDFVSVAPY
PEYFYSNYLALPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Nucleotide

• Length: 1008 bp

>T99381 NZ_CP027550:c3204228-3203221 [Escherichia coli]
ATGGCGAATTGTCGTATTCTGTTAACGCCGTTGAATGAACGTGACGAACAGCGCGGATATTCGACACAGGGGTTGAAGCG
ACTGAGCGGCACTGCAAAATTGACCCCCAGGCTGGGGTTTACGCGAACTCAGTTTGTGCAGGAACTTCCGCGTCAACAAA
AAGGGATGAGTATTTCCGGTTACCAGCCCAAATTGCAATTAGTGCTGGATGAAGGAGAGTTCCGCGTCGTAGATCATCAG
GGAAATTTTATCCTTAAGCCCTCTCCAGCCGACTTTCCCGGCTTGGCCGAAAATGAACACGCAACAATGACATTGATGTC
TCGTTTGGGATTTGACGTACCTGTGCATGGCCTGTTGAGTTTTGCACCACAATCAGAGGAAGAGCTTGAATACGCATTTG
TTATCCGGCGCTATGACCGGGATTACAAGGGGCTGCCAGTGCATCAGGAACAGCTTGATGGCGCGATGCAGATTACAGAT
AAATATGGCAAAACGGGGAATGATAACGAACAATATGTCAGCTATGAAACGCTGGCCCGATTTTTAGTTGCCCATGTTAA
CGACAACATCGCATTTAAAATCGATCTCTTTCGTCGCATTGTTTACGCCTGGTTGCTGGGTAATAACGACATGCATTTAC
GAAACTTTGGTTTAGTGTACTCCGATGGTTTGACGCCAACGTTAGCGCCGGTGTATGACTTTGTTTCTGTCGCTCCCTAT
CCGGAATATTTTTACTCAAACTATCTGGCGCTGCCGTTATTGACGCGAGAAGAAGGCGGTCGGGAACTGGCTCCTGGATT
TCACAGTGATTATGGCGAGTATATCGGGCAGGACTTTTTGCTGTTGGGTGAATCAATGGGGTTAGCGCCCAGATTGCTGG
AAAAACTCTTTCAGGATATACGTAAAGAAAATGCCATTGTTATGGAAACGTATGAGCAGTCCTTTATGACTCAGGATCAT
ATCCAGGCTGTGCTGCAATGCTATCGACATCGTCTGGGGCTGCTGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11822.40 Da
• Isoelectric Point: 6.0609

>AT99381 WP_001490601.1 NZ_CP027550:c3204539-3204228 [Escherichia coli]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAQDYHGPAISISLPVAQRQFPSETLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Nucleotide

• Length: 312 bp

>AT99381 NZ_CP027550:c3204539-3204228 [Escherichia coli]
ATGCATCGACGAGTGAAAGTGTTGCTCTATGGTCAGGTAGTGGGTGAACTCTCTCAGAACGACAGTGGTTTTTTGTTCCA
GTACGCCCAGGATTACCACGGACCGGCAATCTCAATCAGCCTGCCCGTCGCACAGCGTCAGTTTCCCAGTGAGACGTTGC
ATCCTTATTTCGCCTCACTGGCGCCAGAAGGATGGCTGCGTCAACGCTACAGCCAGATACAGCATCGTGACGAAAACGAT
CTGCTGGGAATGCTTATTGATAACGGCAAGAATCTGCTGGGGGCAATCCAGATTTTACCTTGGGAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2A7M399