Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2327099..2327826 Replicon chromosome
Accession NZ_CP027550
Organism Escherichia coli strain 2015C-4136CT1

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag C6950_RS12425 Protein ID WP_000547563.1
Coordinates 2327515..2327826 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A3R1A2R4
Locus tag C6950_RS12420 Protein ID WP_021559999.1
Coordinates 2327099..2327518 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6950_RS12400 2323262..2325514 - 2253 WP_021559998.1 carbamoyltransferase HypF -
C6950_RS12405 2325667..2326194 - 528 WP_001078777.1 electron transport protein HydN -
C6950_RS12415 2326579..2327007 + 429 WP_000536064.1 hypothetical protein -
C6950_RS12420 2327099..2327518 - 420 WP_021559999.1 helix-turn-helix domain-containing protein Antitoxin
C6950_RS12425 2327515..2327826 - 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
C6950_RS12430 2327989..2328744 - 756 Protein_2282 hypothetical protein -
C6950_RS12435 2328787..2329257 - 471 WP_001357572.1 hydrogenase maturation peptidase HycI -
C6950_RS12440 2329250..2329660 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
C6950_RS12445 2329657..2330424 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
C6950_RS12450 2330424..2330966 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
C6950_RS12455 2330976..2332685 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T99376 WP_000547563.1 NZ_CP027550:c2327826-2327515 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T99376 NZ_CP027550:c2327826-2327515 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15446.45 Da        Isoelectric Point: 4.4596

>AT99376 WP_021559999.1 NZ_CP027550:c2327518-2327099 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GAALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT99376 NZ_CP027550:c2327518-2327099 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGCGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure
AlphaFold DB A0A3R1A2R4

References