Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 1452766..1452991 Replicon chromosome
Accession NZ_CP027550
Organism Escherichia coli strain 2015C-4136CT1

Toxin (Protein)


Gene name hokW Uniprot ID S1ELB5
Locus tag C6950_RS07890 Protein ID WP_000813254.1
Coordinates 1452766..1452921 (-) Length 52 a.a.

Antitoxin (RNA)


Gene name sokW
Locus tag -
Coordinates 1452933..1452991 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6950_RS07840 1448895..1449150 - 256 Protein_1444 DUF3927 family protein -
C6950_RS07845 1449147..1449575 - 429 Protein_1445 tellurite resistance TerB family protein -
C6950_RS07870 1450208..1450897 - 690 WP_001064889.1 antiterminator -
C6950_RS07875 1450894..1451259 - 366 WP_021559919.1 RusA family crossover junction endodeoxyribonuclease -
C6950_RS07880 1451260..1452318 - 1059 WP_021559920.1 DUF968 domain-containing protein -
C6950_RS07885 1452320..1452598 - 279 WP_032155008.1 hypothetical protein -
C6950_RS07890 1452766..1452921 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD Toxin
- 1452933..1452991 + 59 - - Antitoxin
C6950_RS07900 1453621..1454064 + 444 WP_021559921.1 acetyltransferase -
C6950_RS07905 1454085..1454669 - 585 WP_021559922.1 XRE family transcriptional regulator -
C6950_RS07910 1454855..1456093 - 1239 WP_021559923.1 IS110 family transposase -
C6950_RS07915 1456409..1456831 - 423 WP_021559924.1 DUF977 family protein -
C6950_RS07920 1456872..1457954 - 1083 WP_021559925.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleF / nleH2 / nleB2 1415134..1469964 54830


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)


Sequences

Toxin        


Download         Length: 52 a.a.        Molecular weight: 5736.89 Da        Isoelectric Point: 6.1531

>T99373 WP_000813254.1 NZ_CP027550:c1452921-1452766 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Download         Length: 156 bp

>T99373 NZ_CP027550:c1452921-1452766 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACTGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 59 bp

>AT99373 NZ_CP027550:1452933-1452991 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TMW0

Antitoxin

Download structure file

References