Detailed information of TA system TA099358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4992309..4993108
• Replicon: chromosome
• Accession: NZ_CP027548
• Organism: Escherichia coli strain 2014C-3061

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: C6992_RS26605
• Protein ID: WP_000347274.1
• Coordinates: 4992309..4992773 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: C6992_RS26610
• Protein ID: WP_001307405.1
• Coordinates: 4992773..4993108 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6992_RS26570	4987310..4987744	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
C6992_RS26575	4987762..4988640	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C6992_RS26580	4988630..4989409	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C6992_RS26585	4989420..4989893	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C6992_RS26590	4989916..4991196	-	1281	WP_032314918.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C6992_RS26600	4991445..4992254	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C6992_RS26605	4992309..4992773	-	465	WP_000347274.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C6992_RS26610	4992773..4993108	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C6992_RS26615	4993257..4994828	-	1572	WP_001273771.1	galactarate dehydratase	-
C6992_RS26620	4995203..4996537	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
C6992_RS26625	4996553..4997323	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17835.22 Da
• Isoelectric Point: 9.4947

>T99358 WP_000347274.1 NZ_CP027548:c4992773-4992309 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLVVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T99358 NZ_CP027548:c4992773-4992309 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGTGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT99358 WP_001307405.1 NZ_CP027548:c4993108-4992773 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT99358 NZ_CP027548:c4993108-4992773 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7