Detailed information of TA system TA099356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4543728..4544330
• Replicon: chromosome
• Accession: NZ_CP027548
• Organism: Escherichia coli strain 2014C-3061

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6992_RS24225
• Protein ID: WP_000897305.1
• Coordinates: 4543728..4544039 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6992_RS24230
• Protein ID: WP_000356397.1
• Coordinates: 4544040..4544330 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6992_RS24200	4539642..4540241	+	600	WP_001295269.1	glucose-1-phosphatase	-
C6992_RS24205	4540235..4541107	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6992_RS24210	4541104..4541541	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6992_RS24215	4541586..4542527	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
C6992_RS24220	4542591..4543499	-	909	WP_001162704.1	alpha/beta hydrolase	-
C6992_RS24225	4543728..4544039	+	312	WP_000897305.1	hypothetical protein	Toxin
C6992_RS24230	4544040..4544330	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6992_RS24240	4544935..4545153	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6992_RS24245	4545372..4545614	+	243	WP_001086388.1	hypothetical protein	-
C6992_RS24250	4545843..4546823	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
C6992_RS24260	4547047..4548300	-	1254	WP_000144344.1	valine--pyruvate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4545843..4546823	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99356 WP_000897305.1 NZ_CP027548:4543728-4544039 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99356 NZ_CP027548:4543728-4544039 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99356 WP_000356397.1 NZ_CP027548:4544040-4544330 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99356 NZ_CP027548:4544040-4544330 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7