Detailed information of TA system TA099345

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1389617..1389988
• Replicon: chromosome
• Accession: NZ_CP027548
• Organism: Escherichia coli strain 2014C-3061

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: C6992_RS07460
• Protein ID: WP_042853000.1
• Coordinates: 1389794..1389988 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1389617..1389795 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6992_RS07420	1385060..1385716	-	657	WP_000011652.1	carbon-nitrogen hydrolase family protein	-
C6992_RS07425	1385818..1386048	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
C6992_RS07430	1386187..1386561	+	375	WP_000168747.1	CopC domain-containing protein YobA	-
C6992_RS07435	1386565..1387436	+	872	Protein_1362	copper homeostasis membrane protein CopD	-
C6992_RS07440	1387449..1387790	+	342	WP_000976476.1	YebY family protein	-
C6992_RS07445	1388183..1389259	-	1077	WP_001189081.1	phage integrase Arm DNA-binding domain-containing protein	-
C6992_RS07450	1389225..1389506	-	282	WP_001311878.1	excisionase	-
C6992_RS07455	1389613..1389801	-	189	WP_001356607.1	DUF1187 family protein	-
-	1389617..1389795	+	179	NuclAT_0	-	Antitoxin
-	1389617..1389795	+	179	NuclAT_0	-	Antitoxin
-	1389617..1389795	+	179	NuclAT_0	-	Antitoxin
-	1389617..1389795	+	179	NuclAT_0	-	Antitoxin
C6992_RS07460	1389794..1389988	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C6992_RS07465	1390045..1390854	-	810	WP_000166311.1	recombination protein RecT	-
C6992_RS07470	1390847..1393594	-	2748	WP_106904308.1	exodeoxyribonuclease VIII	-
C6992_RS07475	1393695..1393972	-	278	Protein_1370	hypothetical protein	-
C6992_RS07480	1394046..1394216	-	171	WP_065336296.1	hypothetical protein	-
C6992_RS07485	1394216..1394437	-	222	WP_000560228.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1382319..1437157	54838

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T99345 WP_042853000.1 NZ_CP027548:c1389988-1389794 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T99345 NZ_CP027548:c1389988-1389794 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACGGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT99345 NZ_CP027548:1389617-1389795 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin