Detailed information of TA system TA099333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 278315..279042
• Replicon: chromosome
• Accession: NZ_CP027548
• Organism: Escherichia coli strain 2014C-3061

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: C6992_RS01440
• Protein ID: WP_000547563.1
• Coordinates: 278315..278626 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6992_RS01445
• Protein ID: WP_000126296.1
• Coordinates: 278623..279042 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6992_RS01410	273458..275167	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
C6992_RS01415	275177..275719	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
C6992_RS01420	275719..276486	+	768	WP_106904283.1	formate hydrogenlyase subunit HycG	-
C6992_RS01425	276483..276893	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C6992_RS01430	276886..277356	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C6992_RS01435	277381..278142	+	762	WP_001026448.1	hypothetical protein	-
C6992_RS01440	278315..278626	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6992_RS01445	278623..279042	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
C6992_RS01450	279156..280580	-	1425	WP_000110373.1	6-phospho-beta-glucosidase AscB	-
C6992_RS01455	280589..282046	-	1458	Protein_271	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C6992_RS01460	282306..283316	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
C6992_RS01465	283465..283992	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T99333 WP_000547563.1 NZ_CP027548:278315-278626 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T99333 NZ_CP027548:278315-278626 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT99333 WP_000126296.1 NZ_CP027548:278623-279042 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT99333 NZ_CP027548:278623-279042 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22