Detailed information of TA system TA099325

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4580311..4580723
• Replicon: chromosome
• Accession: NZ_CP027546
• Organism: Escherichia coli strain 2013C-4187

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A0F3WCK2
• Locus tag: C6993_RS24870
• Protein ID: WP_000132621.1
• Coordinates: 4580311..4580652 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4580647..4580723 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6993_RS24855	4576321..4577733	-	1413	WP_000199352.1	PLP-dependent aminotransferase family protein	-
C6993_RS24860	4577910..4578074	+	165	WP_000394276.1	DUF1127 domain-containing protein	-
C6993_RS24865	4578171..4580264	+	2094	WP_000648228.1	DUF262 domain-containing protein	-
C6993_RS24870	4580311..4580652	-	342	WP_000132621.1	endoribonuclease SymE	Toxin
-	4580647..4580723	+	77	NuclAT_27	-	Antitoxin
-	4580647..4580723	+	77	NuclAT_27	-	Antitoxin
-	4580647..4580723	+	77	NuclAT_27	-	Antitoxin
-	4580647..4580723	+	77	NuclAT_27	-	Antitoxin
-	4580647..4580723	+	77	NuclAT_28	-	Antitoxin
-	4580647..4580723	+	77	NuclAT_28	-	Antitoxin
-	4580647..4580723	+	77	NuclAT_28	-	Antitoxin
-	4580647..4580723	+	77	NuclAT_28	-	Antitoxin
C6993_RS24875	4580873..4582642	-	1770	WP_000110079.1	restriction endonuclease subunit S	-
C6993_RS24880	4582642..4584111	-	1470	WP_001341289.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12342.12 Da
• Isoelectric Point: 7.8218

>T99325 WP_000132621.1 NZ_CP027546:c4580652-4580311 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESE
LMQSLRQVCKLSARKQKQVQDFIGVITGKQKVA

Nucleotide

• Length: 342 bp

>T99325 NZ_CP027546:c4580652-4580311 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCACCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTAATTAC
CGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT99325 NZ_CP027546:4580647-4580723 [Escherichia coli]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0F3WCK2

Antitoxin