Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 5167919..5168421 Replicon chromosome
Accession NZ_CP027544
Organism Escherichia coli strain 2013C-3264

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MJ68_RS27785 Protein ID WP_000767829.1
Coordinates 5168167..5168421 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MJ68_RS27780 Protein ID WP_001259255.1
Coordinates 5167919..5168170 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ68_RS27750 5163097..5164163 - 1067 Protein_5031 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
MJ68_RS27755 5164163..5165233 - 1071 WP_000108962.1 histidinol-phosphate transaminase -
MJ68_RS27760 5165230..5166534 - 1305 WP_001397240.1 histidinol dehydrogenase -
MJ68_RS27765 5166540..5167439 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
MJ68_RS27775 5167585..5167635 - 51 WP_001364200.1 his operon leader peptide -
MJ68_RS27780 5167919..5168170 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MJ68_RS27785 5168167..5168421 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MJ68_RS27790 5168504..5169328 + 825 WP_000754752.1 SDR family oxidoreductase -
MJ68_RS27795 5169374..5170303 + 930 WP_000803351.1 LysR family transcriptional regulator -
MJ68_RS27800 5170518..5170580 + 63 WP_010723108.1 membrane protein YoeI -
MJ68_RS27805 5170570..5171928 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MJ68_RS27810 5172107..5173165 + 1059 WP_000492346.1 transport protein YeeE -
MJ68_RS27815 5173179..5173406 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99291 WP_000767829.1 NZ_CP027544:5168167-5168421 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99291 NZ_CP027544:5168167-5168421 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99291 WP_001259255.1 NZ_CP027544:5167919-5168170 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99291 NZ_CP027544:5167919-5168170 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References