Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4126850..4127391 Replicon chromosome
Accession NZ_CP027544
Organism Escherichia coli strain 2013C-3264

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag MJ68_RS22330 Protein ID WP_000615976.1
Coordinates 4126850..4127128 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag MJ68_RS22335 Protein ID WP_000729704.1
Coordinates 4127131..4127391 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ68_RS22315 4124432..4125010 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
MJ68_RS22320 4125216..4125983 + 768 WP_001365365.1 class II glutamine amidotransferase -
MJ68_RS22325 4125954..4126694 - 741 WP_001225679.1 murein L,D-transpeptidase -
MJ68_RS22330 4126850..4127128 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MJ68_RS22335 4127131..4127391 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MJ68_RS22340 4127577..4128350 + 774 WP_000543903.1 C40 family peptidase -
MJ68_RS22345 4128407..4128763 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
MJ68_RS22350 4128756..4129034 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
MJ68_RS22355 4129139..4130851 - 1713 Protein_4051 flagellar biosynthesis protein FlhA -
MJ68_RS22360 4130823..4131608 + 786 WP_000207555.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T99288 WP_000615976.1 NZ_CP027544:c4127128-4126850 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T99288 NZ_CP027544:c4127128-4126850 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT99288 WP_000729704.1 NZ_CP027544:c4127391-4127131 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT99288 NZ_CP027544:c4127391-4127131 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References