Detailed information of TA system TA099283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3148429..3149031
• Replicon: chromosome
• Accession: NZ_CP027544
• Organism: Escherichia coli strain 2013C-3264

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MJ68_RS17290
• Protein ID: WP_000897305.1
• Coordinates: 3148429..3148740 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJ68_RS17295
• Protein ID: WP_000356397.1
• Coordinates: 3148741..3149031 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ68_RS17265	3144343..3144942	+	600	WP_001295269.1	glucose-1-phosphatase	-
MJ68_RS17270	3144936..3145808	+	873	WP_000920762.1	virulence factor BrkB family protein	-
MJ68_RS17275	3145805..3146242	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MJ68_RS17280	3146287..3147228	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
MJ68_RS17285	3147292..3148200	-	909	WP_001162704.1	alpha/beta hydrolase	-
MJ68_RS17290	3148429..3148740	+	312	WP_000897305.1	hypothetical protein	Toxin
MJ68_RS17295	3148741..3149031	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
MJ68_RS17305	3149636..3149854	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
MJ68_RS17310	3150073..3150315	+	243	WP_001086388.1	hypothetical protein	-
MJ68_RS17315	3150544..3151524	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
MJ68_RS17325	3151924..3152853	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
MJ68_RS17330	3152850..3153485	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3150544..3151524	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99283 WP_000897305.1 NZ_CP027544:3148429-3148740 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99283 NZ_CP027544:3148429-3148740 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99283 WP_000356397.1 NZ_CP027544:3148741-3149031 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99283 NZ_CP027544:3148741-3149031 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7