Detailed information of TA system TA099281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2348741..2349540
• Replicon: chromosome
• Accession: NZ_CP027544
• Organism: Escherichia coli strain 2013C-3264

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: MJ68_RS13125
• Protein ID: WP_000347274.1
• Coordinates: 2349076..2349540 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: MJ68_RS13120
• Protein ID: WP_001307405.1
• Coordinates: 2348741..2349076 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ68_RS13105	2344526..2345296	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
MJ68_RS13110	2345312..2346646	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
MJ68_RS13115	2347021..2348592	+	1572	WP_001273771.1	galactarate dehydratase	-
MJ68_RS13120	2348741..2349076	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
MJ68_RS13125	2349076..2349540	+	465	WP_000347274.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
MJ68_RS13130	2349595..2350404	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
MJ68_RS13140	2350653..2351933	+	1281	WP_000681922.1	tagatose-bisphosphate aldolase subunit KbaZ	-
MJ68_RS13145	2351956..2352429	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
MJ68_RS13150	2352440..2353219	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
MJ68_RS13155	2353209..2354087	+	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
MJ68_RS13160	2354105..2354539	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2336701..2349540	12839

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17835.22 Da
• Isoelectric Point: 9.4947

>T99281 WP_000347274.1 NZ_CP027544:2349076-2349540 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLVVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T99281 NZ_CP027544:2349076-2349540 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGTGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT99281 WP_001307405.1 NZ_CP027544:2348741-2349076 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT99281 NZ_CP027544:2348741-2349076 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTACCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7