Detailed information of TA system TA099276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1738999..1739726
• Replicon: chromosome
• Accession: NZ_CP027544
• Organism: Escherichia coli strain 2013C-3264

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: MJ68_RS09860
• Protein ID: WP_000547563.1
• Coordinates: 1739415..1739726 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJ68_RS09855
• Protein ID: WP_000126296.1
• Coordinates: 1738999..1739418 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ68_RS09835	1734049..1734576	-	528	WP_001078777.1	electron transport protein HydN	-
MJ68_RS09840	1734725..1735735	-	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
MJ68_RS09845	1735995..1737452	+	1458	WP_001107872.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
MJ68_RS09850	1737461..1738885	+	1425	WP_000110373.1	6-phospho-beta-glucosidase AscB	-
MJ68_RS09855	1738999..1739418	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
MJ68_RS09860	1739415..1739726	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
MJ68_RS09865	1739899..1740660	-	762	WP_001026448.1	hypothetical protein	-
MJ68_RS09870	1740685..1741155	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
MJ68_RS09875	1741148..1741558	-	411	WP_001291931.1	formate hydrogenlyase assembly protein HycH	-
MJ68_RS09880	1741555..1742322	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
MJ68_RS09885	1742322..1742864	-	543	WP_000493790.1	formate hydrogenlyase subunit HycF	-
MJ68_RS09890	1742874..1744583	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T99276 WP_000547563.1 NZ_CP027544:c1739726-1739415 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T99276 NZ_CP027544:c1739726-1739415 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT99276 WP_000126296.1 NZ_CP027544:c1739418-1738999 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT99276 NZ_CP027544:c1739418-1738999 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22