Detailed information of TA system TA099240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4732169..4732850
• Replicon: chromosome
• Accession: NZ_CP027538
• Organism: Pseudomonas aeruginosa strain AR_0095

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM474_RS22755
• Protein ID: WP_034028006.1
• Coordinates: 4732169..4732534 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM474_RS22760
• Protein ID: WP_003145733.1
• Coordinates: 4732527..4732850 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM474_RS22725	4728170..4728604	-	435	WP_034028004.1	RidA family protein	-
AM474_RS22730	4728825..4729778	+	954	WP_003140893.1	LysR family transcriptional regulator	-
AM474_RS22735	4729751..4730635	-	885	WP_009875989.1	LysR family transcriptional regulator	-
AM474_RS22740	4730736..4731161	+	426	WP_003111827.1	ring-cleaving dioxygenase	-
AM474_RS22745	4731192..4731464	-	273	WP_003124230.1	hypothetical protein	-
AM474_RS22750	4731671..4731910	+	240	WP_044060930.1	hypothetical protein	-
AM474_RS22755	4732169..4732534	+	366	WP_034028006.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM474_RS22760	4732527..4732850	+	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
AM474_RS22765	4733226..4734314	-	1089	WP_003140888.1	DUF3396 domain-containing protein	-
AM474_RS22770	4734331..4735032	-	702	WP_003111822.1	VRR-NUC domain-containing protein	-
AM474_RS22775	4735029..4735547	-	519	WP_003140886.1	PAAR domain-containing protein	-
AM474_RS22780	4735721..4736056	-	336	WP_003140884.1	TM2 domain-containing protein	-
AM474_RS22785	4736299..4736937	-	639	WP_003140882.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4712401..4736056	23655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13838.16 Da
• Isoelectric Point: 4.9885

>T99240 WP_034028006.1 NZ_CP027538:4732169-4732534 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T99240 NZ_CP027538:4732169-4732534 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGAGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT99240 WP_003145733.1 NZ_CP027538:4732527-4732850 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT99240 NZ_CP027538:4732527-4732850 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATCGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA