Detailed information of TA system TA099232

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66978..67217
• Replicon: plasmid unnamed2
• Accession: NZ_CP027537
• Organism: Escherichia coli strain AR_0081

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: AM460_RS27950
• Protein ID: WP_023144756.1
• Coordinates: 66978..67112 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	67157..67217 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM460_RS27915	62989..63390	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
AM460_RS28410	63323..63580	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
AM460_RS27920	63673..64326	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
AM460_RS27930	65266..66123	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
AM460_RS27935	66116..66190	-	75	WP_031943482.1	RepA leader peptide Tap	-
AM460_RS28620	66187..66321	-	135	Protein_82	protein CopA/IncA	-
AM460_RS27945	66427..66681	-	255	WP_000083850.1	replication regulatory protein RepA	-
AM460_RS27950	66978..67112	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	67157..67217	+	61	NuclAT_2	-	Antitoxin
-	67157..67217	+	61	NuclAT_2	-	Antitoxin
-	67157..67217	+	61	NuclAT_2	-	Antitoxin
-	67157..67217	+	61	NuclAT_2	-	Antitoxin
AM460_RS28625	67184..67470	-	287	Protein_85	DUF2726 domain-containing protein	-
AM460_RS27960	67548..69161	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
AM460_RS27965	69192..69542	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
AM460_RS27970	69539..69964	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
AM460_RS27980	70523..70735	-	213	WP_013023861.1	hypothetical protein	-
AM460_RS27985	70866..71426	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..100279	100279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T99232 WP_023144756.1 NZ_CP027537:c67112-66978 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T99232 NZ_CP027537:c67112-66978 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT99232 NZ_CP027537:67157-67217 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin