Detailed information of TA system TA099226

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 8517..8759
• Replicon: plasmid unnamed2
• Accession: NZ_CP027537
• Organism: Escherichia coli strain AR_0081

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM460_RS27560
• Protein ID: WP_001312861.1
• Coordinates: 8517..8675 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	8719..8759 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM460_RS27515	3628..3855	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
AM460_RS27520	3943..4620	-	678	WP_001348626.1	PAS domain-containing protein	-
AM460_RS27525	4754..5137	-	384	WP_001151566.1	relaxosome protein TraM	-
AM460_RS27530	5468..6070	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
AM460_RS27535	6367..7188	-	822	WP_001234469.1	DUF945 domain-containing protein	-
AM460_RS27540	7308..7595	-	288	WP_000107535.1	hypothetical protein	-
AM460_RS28605	7620..7826	-	207	WP_000275859.1	hypothetical protein	-
AM460_RS28610	7739..8074	-	336	WP_013023876.1	hypothetical protein	-
AM460_RS27560	8517..8675	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	8719..8759	-	41	NuclAT_1	-	Antitoxin
-	8719..8759	-	41	NuclAT_1	-	Antitoxin
-	8719..8759	-	41	NuclAT_1	-	Antitoxin
-	8719..8759	-	41	NuclAT_1	-	Antitoxin
-	10203..10389	-	187	NuclAT_0	-	-
-	10203..10389	-	187	NuclAT_0	-	-
-	10203..10389	-	187	NuclAT_0	-	-
-	10203..10389	-	187	NuclAT_0	-	-
AM460_RS27570	10401..11120	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
AM460_RS27575	11117..11551	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
AM460_RS27580	11606..13564	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..100279	100279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T99226 WP_001312861.1 NZ_CP027537:c8675-8517 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T99226 NZ_CP027537:c8675-8517 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT99226 NZ_CP027537:c8759-8719 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin