Detailed information of TA system TA099219

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19056..19482
• Replicon: plasmid unnamed3
• Accession: NZ_CP027536
• Organism: Escherichia coli strain AR_0081

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM460_RS27140
• Protein ID: WP_001312861.1
• Coordinates: 19056..19214 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19258..19482 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM460_RS27100	14433..15122	-	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-
AM460_RS27105	15309..15692	-	384	WP_001151538.1	relaxosome protein TraM	-
AM460_RS27110	16013..16615	+	603	Protein_23	transglycosylase SLT domain-containing protein	-
AM460_RS27115	16912..17733	-	822	WP_001234445.1	DUF945 domain-containing protein	-
AM460_RS27120	17844..18140	-	297	WP_001272251.1	hypothetical protein	-
AM460_RS27140	19056..19214	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	19258..19482	-	225	NuclAT_0	-	Antitoxin
-	19258..19482	-	225	NuclAT_0	-	Antitoxin
-	19258..19482	-	225	NuclAT_0	-	Antitoxin
-	19258..19482	-	225	NuclAT_0	-	Antitoxin
AM460_RS28365	19294..19473	+	180	WP_001309233.1	hypothetical protein	-
AM460_RS27145	19494..20213	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
AM460_RS27150	20210..20644	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
AM460_RS27155	20713..22677	-	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
AM460_RS27160	22738..22971	-	234	WP_000006003.1	DUF905 family protein	-
AM460_RS27165	23029..23556	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..72482	72482

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T99219 WP_001312861.1 NZ_CP027536:c19214-19056 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T99219 NZ_CP027536:c19214-19056 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT99219 NZ_CP027536:c19482-19258 [Escherichia coli]
TCGCACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin