Detailed information of TA system TA099181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3011551..3012211
• Replicon: chromosome
• Accession: NZ_CP027533
• Organism: Serratia marcescens strain AR_0091

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM470_RS14660
• Protein ID: WP_043147691.1
• Coordinates: 3011858..3012211 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2V4GQ69
• Locus tag: AM470_RS14655
• Protein ID: WP_033635213.1
• Coordinates: 3011551..3011853 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM470_RS14635	3007181..3008095	-	915	WP_004928592.1	branched-chain amino acid ABC transporter permease	-
AM470_RS14640	3008221..3009339	-	1119	WP_033647954.1	branched-chain amino acid ABC transporter substrate-binding protein	-
AM470_RS14650	3009943..3011151	-	1209	WP_043147690.1	aldose 1-epimerase family protein	-
AM470_RS14655	3011551..3011853	-	303	WP_033635213.1	XRE family transcriptional regulator	Antitoxin
AM470_RS14660	3011858..3012211	-	354	WP_043147691.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM470_RS14665	3012651..3013946	+	1296	WP_043147692.1	MFS transporter	-
AM470_RS14670	3013974..3014780	+	807	WP_043147694.1	substrate-binding domain-containing protein	-
AM470_RS14675	3014755..3015654	-	900	WP_033647959.1	LysR family transcriptional regulator	-
AM470_RS14680	3015747..3016112	-	366	WP_033635218.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13432.24 Da
• Isoelectric Point: 7.9895

>T99181 WP_043147691.1 NZ_CP027533:c3012211-3011858 [Serratia marcescens]
VWEIKTTDAFDNWFSSLHDADRAGVLAALMILREKGPGLPRPYADTVKGSRYSNMKELRIQSRGEPLRAFFAFDPYRIGI
VLCAGNKAGNEKRFYDQMIQAADREFTNWLNTLNEKE

Nucleotide

• Length: 354 bp

>T99181 NZ_CP027533:c3012211-3011858 [Serratia marcescens]
GTGTGGGAAATTAAAACAACGGATGCGTTTGATAACTGGTTTAGCTCACTCCACGATGCCGATCGGGCTGGCGTGCTTGC
CGCATTGATGATCCTGCGGGAAAAGGGGCCGGGGCTGCCCAGACCCTATGCCGATACGGTTAAAGGTTCGCGTTACAGCA
ATATGAAAGAGCTGCGCATTCAGAGCCGTGGCGAACCGTTGCGGGCGTTCTTCGCCTTCGATCCGTACCGTATCGGCATT
GTACTTTGCGCGGGCAATAAAGCCGGTAATGAAAAACGTTTTTACGATCAGATGATTCAGGCTGCCGATCGGGAATTCAC
GAACTGGCTGAACACATTAAACGAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11079.94 Da
• Isoelectric Point: 6.4738

>AT99181 WP_033635213.1 NZ_CP027533:c3011853-3011551 [Serratia marcescens]
MGRTLEQLIADEKPEVVAKAQALAADIMLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT99181 NZ_CP027533:c3011853-3011551 [Serratia marcescens]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAGCCCGAGGTCGTGGCCAAAGCGCAAGCGCTGGCGGCTGACAT
CATGCTGAACATCCACCTTGCCGAGCTGCGCGAGAAGGTGCAGAAAACGCAGGTTGAAATGGCGCAGGCGCTGGGTATCC
GGCAACCGACCGTTGCCGTGATGGAAAAACCGGGCCGCGATCTGAAACTGTCTTCGCTAAAACGCTATGTTGAGGCGGCC
GGCGGCAAGCTGCGCCTGGATGTTGAACTGCCGGACGGCTCTCACTACGAATTTGTGCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2V4GQ69