Detailed information of TA system TA099168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 78795..79380
• Replicon: plasmid unnamed1
• Accession: NZ_CP027531
• Organism: Acinetobacter baumannii strain AR_0088

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7S7BY50
• Locus tag: AM467_RS19940
• Protein ID: WP_000897311.1
• Coordinates: 78795..79115 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM467_RS19945
• Protein ID: WP_000369784.1
• Coordinates: 79108..79380 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM467_RS19910	74602..74931	+	330	WP_000506885.1	DUF4236 domain-containing protein	-
AM467_RS19920	75203..76435	-	1233	WP_001255535.1	ATP-binding protein	-
AM467_RS19930	76693..77130	-	438	Protein_75	IS3 family transposase	-
AM467_RS19935	77184..78116	+	933	WP_000743272.1	IS5-like element IS17 family transposase	-
AM467_RS19940	78795..79115	+	321	WP_000897311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM467_RS19945	79108..79380	+	273	WP_000369784.1	helix-turn-helix domain-containing protein	Antitoxin
AM467_RS19950	79413..80213	+	801	WP_000604996.1	DUF2971 domain-containing protein	-
AM467_RS19955	80304..80605	+	302	Protein_80	transposase	-
AM467_RS19960	80721..80933	+	213	WP_001123161.1	hypothetical protein	-
AM467_RS19965	81259..82116	-	858	WP_000609033.1	LysR family transcriptional regulator	-
AM467_RS19970	82113..82514	-	402	Protein_83	hydroxyacid dehydrogenase	-
AM467_RS19975	82579..83283	+	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
AM467_RS19985	83334..83513	-	180	Protein_85	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	aac(3)-IIa / aac(6')-Ian	-	76693..89610	12917
	-	inside	Non-Mobilizable plasmid	sul2 / tet(B) / aph(6)-Id / aph(3'')-Ib / aac(3)-IIa / aac(6')-Ian	-	1..146698	146698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12214.03 Da
• Isoelectric Point: 7.9500

>T99168 WP_000897311.1 NZ_CP027531:78795-79115 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFNG

Nucleotide

• Length: 321 bp

>T99168 NZ_CP027531:78795-79115 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTTACTGATGAAGAATATCGTCAACTCCAGCA
AGATCTATTGATACAACCTGATAAAGGAGACTTGATCAAAAATGGTGGTGGCATTCGTAAAGTCCGTTGTGCTCAGGGTA
ATAAAGGCAAAAGTGGCGGTATTCGTGTGATCTATTACTGGGTTACAGAGGATGATCAGATTTTTTTCCTAGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTAATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10145.57 Da
• Isoelectric Point: 7.0146

>AT99168 WP_000369784.1 NZ_CP027531:79108-79380 [Acinetobacter baumannii]
MDNNLFDDLVTSIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDTNPTLI

Nucleotide

• Length: 273 bp

>AT99168 NZ_CP027531:79108-79380 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTCGTTACTTCCATCAAAGAAGCTGGAGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTAACAGAACTTGAATTGCCTGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCAGAGTTTGCTGCAC
GCTTACATATTAGCGCGAGAACTCTACAAAATTGGGAACAAGGTCGTCGCTATCCGACTGGCCCAGCAGCGACTTTGATT
CGTATTCTTGATACCAATCCAACTCTGATTTAG

Structures

Toxin

• AlphaFold DB: A0A7S7BY50