Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 244106..244641 Replicon plasmid unnamed1
Accession NZ_CP027518
Organism Enterococcus faecium strain AUSMDU00004024

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag C6Y19_RS16120 Protein ID WP_002314366.1
Coordinates 244106..244378 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag C6Y19_RS16125 Protein ID WP_002297422.1
Coordinates 244378..244641 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6Y19_RS16075 239212..239661 - 450 WP_010729386.1 TlpA family protein disulfide reductase -
C6Y19_RS16700 239817..240359 - 543 WP_002298088.1 hypothetical protein -
C6Y19_RS16090 240634..241277 + 644 Protein_269 transposase -
C6Y19_RS16095 241353..242033 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
C6Y19_RS16100 242092..242280 - 189 Protein_271 hypothetical protein -
C6Y19_RS16105 242281..243138 - 858 WP_002292680.1 AAA family ATPase -
C6Y19_RS16110 243424..243710 - 287 Protein_273 type II toxin-antitoxin system RelE/ParE family toxin -
C6Y19_RS16115 243700..244029 - 330 WP_002292678.1 AbrB family transcriptional regulator -
C6Y19_RS16120 244106..244378 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
C6Y19_RS16125 244378..244641 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C6Y19_RS16130 244875..245782 + 908 Protein_277 IS982 family transposase -
C6Y19_RS16135 245785..246960 - 1176 WP_002336205.1 MFS transporter -
C6Y19_RS16140 246963..249212 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..259384 259384
- inside IScluster/Tn - - 232543..257132 24589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T99154 WP_002314366.1 NZ_CP027518:c244378-244106 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T99154 NZ_CP027518:c244378-244106 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT99154 WP_002297422.1 NZ_CP027518:c244641-244378 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT99154 NZ_CP027518:c244641-244378 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References