Detailed information of TA system TA099123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4502448..4503050
• Replicon: chromosome
• Accession: NZ_CP027472
• Organism: Escherichia coli strain 2014C-3050

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AAF93_RS24410
• Protein ID: WP_000897305.1
• Coordinates: 4502448..4502759 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AAF93_RS24415
• Protein ID: WP_000356397.1
• Coordinates: 4502760..4503050 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAF93_RS24380	4497478..4498263	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AAF93_RS24385	4498362..4498961	+	600	WP_001315111.1	glucose-1-phosphatase	-
AAF93_RS24390	4498955..4499827	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AAF93_RS24395	4499824..4500261	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AAF93_RS24400	4500306..4501247	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AAF93_RS24405	4501311..4502219	-	909	WP_001162704.1	alpha/beta hydrolase	-
AAF93_RS24410	4502448..4502759	+	312	WP_000897305.1	hypothetical protein	Toxin
AAF93_RS24415	4502760..4503050	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AAF93_RS24425	4503656..4503874	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
AAF93_RS24430	4504093..4504335	+	243	WP_001086390.1	hypothetical protein	-
AAF93_RS24435	4504564..4505544	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
AAF93_RS24445	4505944..4506873	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AAF93_RS24450	4506870..4507505	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4504564..4505544	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99123 WP_000897305.1 NZ_CP027472:4502448-4502759 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99123 NZ_CP027472:4502448-4502759 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99123 WP_000356397.1 NZ_CP027472:4502760-4503050 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99123 NZ_CP027472:4502760-4503050 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7