Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3249066..3249601 Replicon chromosome
Accession NZ_CP027472
Organism Escherichia coli strain 2014C-3050

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag AAF93_RS18040 Protein ID WP_032183258.1
Coordinates 3249314..3249601 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AAF93_RS18035 Protein ID WP_000729704.1
Coordinates 3249066..3249326 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF93_RS18010 3244102..3245241 + 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -
AAF93_RS18015 3245225..3247318 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
AAF93_RS18020 3247423..3247701 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AAF93_RS18025 3247694..3248050 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AAF93_RS18030 3248107..3248880 - 774 WP_000543897.1 C40 family peptidase -
AAF93_RS18035 3249066..3249326 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AAF93_RS18040 3249314..3249601 + 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AAF93_RS18045 3249757..3250497 + 741 WP_001225679.1 murein L,D-transpeptidase -
AAF93_RS18050 3250468..3251235 - 768 WP_000333380.1 class II glutamine amidotransferase -
AAF93_RS18055 3251441..3252019 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T99119 WP_032183258.1 NZ_CP027472:3249314-3249601 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T99119 NZ_CP027472:3249314-3249601 [Escherichia coli]
ATTAGGAATTTAAATCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAA
GGATATGAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACC
CGCTTCAAGGTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAA
CTTTTACGATTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT99119 WP_000729704.1 NZ_CP027472:3249066-3249326 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT99119 NZ_CP027472:3249066-3249326 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References