Detailed information of TA system TA099106

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 978769..979140
• Replicon: chromosome
• Accession: NZ_CP027472
• Organism: Escherichia coli strain 2014C-3050

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: AAF93_RS05065
• Protein ID: WP_042853000.1
• Coordinates: 978946..979140 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	978769..978947 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAF93_RS05025	974211..974867	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-
AAF93_RS05030	974969..975199	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
AAF93_RS05035	975338..975712	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
AAF93_RS05040	975716..976588	+	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
AAF93_RS05045	976601..976942	+	342	WP_000976492.1	YebY family protein	-
AAF93_RS05050	977335..978411	-	1077	WP_001189091.1	phage integrase Arm DNA-binding domain-containing protein	-
AAF93_RS05055	978377..978658	-	282	WP_001443927.1	excisionase	-
AAF93_RS05060	978765..978953	-	189	WP_001356607.1	DUF1187 family protein	-
-	978769..978947	+	179	NuclAT_1	-	Antitoxin
-	978769..978947	+	179	NuclAT_1	-	Antitoxin
-	978769..978947	+	179	NuclAT_1	-	Antitoxin
-	978769..978947	+	179	NuclAT_1	-	Antitoxin
AAF93_RS05065	978946..979140	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
AAF93_RS05070	979197..980007	-	811	Protein_937	recombination protein RecT	-
AAF93_RS05075	980000..982651	-	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
AAF93_RS05080	982750..983025	-	276	WP_001307773.1	hypothetical protein	-
AAF93_RS05085	983099..983275	-	177	WP_000245534.1	hypothetical protein	-
AAF93_RS05090	983269..983490	-	222	WP_000560218.1	killing protein KilR	-
AAF93_RS05095	983911..984063	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	971468..1026898	55430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T99106 WP_042853000.1 NZ_CP027472:c979140-978946 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T99106 NZ_CP027472:c979140-978946 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT99106 NZ_CP027472:978769-978947 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin