Detailed information of TA system TA099069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4539990..4540592
• Replicon: chromosome
• Accession: NZ_CP027461
• Organism: Escherichia coli strain 95-3322

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: C6W78_RS23525
• Protein ID: WP_000897302.1
• Coordinates: 4539990..4540301 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6W78_RS23530
• Protein ID: WP_000356397.1
• Coordinates: 4540302..4540592 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W78_RS23495	4535020..4535805	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
C6W78_RS23500	4535904..4536503	+	600	WP_001298594.1	glucose-1-phosphatase	-
C6W78_RS23505	4536497..4537369	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6W78_RS23510	4537366..4537803	+	438	WP_000560990.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6W78_RS23515	4537848..4538789	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6W78_RS23520	4538853..4539761	-	909	WP_001305059.1	alpha/beta hydrolase	-
C6W78_RS23525	4539990..4540301	+	312	WP_000897302.1	hypothetical protein	Toxin
C6W78_RS23530	4540302..4540592	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6W78_RS23540	4540951..4541229	+	279	WP_001296612.1	hypothetical protein	-
C6W78_RS23550	4541625..4541843	+	219	WP_159032323.1	ribbon-helix-helix domain-containing protein	-
C6W78_RS23555	4542059..4542988	-	930	WP_042021934.1	formate dehydrogenase accessory protein FdhE	-
C6W78_RS23560	4542985..4543620	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6W78_RS23565	4543617..4544519	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T99069 WP_000897302.1 NZ_CP027461:4539990-4540301 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99069 NZ_CP027461:4539990-4540301 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99069 WP_000356397.1 NZ_CP027461:4540302-4540592 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99069 NZ_CP027461:4540302-4540592 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416