99057

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1762501..1763063 Replicon chromosome
Accession NZ_CP027461
Organism Escherichia coli strain 95-3322

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag C6W78_RS09385 Protein ID WP_000605675.1
Coordinates 1762785..1763063 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag C6W78_RS09380 Protein ID WP_000781370.1
Coordinates 1762501..1762785 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W78_RS09365 1757682..1760729 + 3048 WP_077818373.1 formate dehydrogenase-N subunit alpha -
C6W78_RS09370 1760742..1761626 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
C6W78_RS09375 1761619..1762272 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
C6W78_RS09380 1762501..1762785 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
C6W78_RS09385 1762785..1763063 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6W78_RS09390 1763249..1764259 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
C6W78_RS09395 1764393..1766090 - 1698 WP_000433460.1 malate dehydrogenase -
C6W78_RS09400 1766247..1766384 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
C6W78_RS09405 1766486..1766701 - 216 WP_042021064.1 biofilm-dependent modulation protein -
C6W78_RS09415 1767046..1767477 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T99057 WP_000605675.1 NZ_CP027461:c1763063-1762785 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T99057 NZ_CP027461:c1763063-1762785 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT99057 WP_000781370.1 NZ_CP027461:c1762785-1762501 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT99057 NZ_CP027461:c1762785-1762501 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References