Detailed information of TA system TA099050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 407890..408584
• Replicon: chromosome
• Accession: NZ_CP027461
• Organism: Escherichia coli strain 95-3322

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: C6W78_RS02070
• Protein ID: WP_042020796.1
• Coordinates: 408186..408584 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: C6W78_RS02065
• Protein ID: WP_000554758.1
• Coordinates: 407890..408183 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W78_RS02040	403511..403867	-	357	WP_001030482.1	type II toxin-antitoxin system HicB family antitoxin	-
C6W78_RS02045	403860..404138	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
C6W78_RS02050	404243..405955	-	1713	Protein_380	flagellar biosynthesis protein FlhA	-
C6W78_RS02055	405927..406712	+	786	WP_172906057.1	putative lateral flagellar export/assembly protein LafU	-
C6W78_RS02060	406783..407838	+	1056	WP_001226168.1	DNA polymerase IV	-
C6W78_RS02065	407890..408183	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C6W78_RS02070	408186..408584	+	399	WP_042020796.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C6W78_RS02075	408594..409046	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
C6W78_RS02080	409237..410376	+	1140	WP_001356952.1	RNA ligase RtcB family protein	-
C6W78_RS02085	410373..410987	+	615	WP_000602123.1	peptide chain release factor H	-
C6W78_RS02090	411044..412501	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
C6W78_RS02095	412762..413220	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	387849..409046	21197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15381.79 Da
• Isoelectric Point: 8.9511

>T99050 WP_042020796.1 NZ_CP027461:408186-408584 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKVAEAFRMRF

Nucleotide

• Length: 399 bp

>T99050 NZ_CP027461:408186-408584 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAGTGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT99050 WP_000554758.1 NZ_CP027461:407890-408183 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT99050 NZ_CP027461:407890-408183 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1QAE3