Detailed information of TA system TA099041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4744505..4745304
• Replicon: chromosome
• Accession: NZ_CP027459
• Organism: Escherichia coli strain 90-3040

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: C6W76_RS25550
• Protein ID: WP_032208138.1
• Coordinates: 4744505..4744969 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: C6W76_RS25555
• Protein ID: WP_032208136.1
• Coordinates: 4744969..4745304 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W76_RS25515	4739506..4739940	-	435	WP_032208141.1	PTS sugar transporter subunit IIA	-
C6W76_RS25520	4739958..4740836	-	879	WP_001341890.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C6W76_RS25525	4740826..4741605	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C6W76_RS25530	4741616..4742089	-	474	WP_032208140.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C6W76_RS25535	4742112..4743392	-	1281	WP_047087618.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C6W76_RS25545	4743641..4744450	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C6W76_RS25550	4744505..4744969	-	465	WP_032208138.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C6W76_RS25555	4744969..4745304	-	336	WP_032208136.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C6W76_RS25560	4745453..4747024	-	1572	WP_032208135.1	galactarate dehydratase	-
C6W76_RS25565	4747399..4748733	+	1335	WP_047087619.1	galactarate/glucarate/glycerate transporter GarP	-
C6W76_RS25570	4748749..4749519	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17837.19 Da
• Isoelectric Point: 9.4947

>T99041 WP_032208138.1 NZ_CP027459:c4744969-4744505 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGASRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T99041 NZ_CP027459:c4744969-4744505 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTAGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12405.01 Da
• Isoelectric Point: 4.8616

>AT99041 WP_032208136.1 NZ_CP027459:c4745304-4744969 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKLGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT99041 NZ_CP027459:c4745304-4744969 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCTAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA