Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3056307..3056848 Replicon chromosome
Accession NZ_CP027459
Organism Escherichia coli strain 90-3040

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag C6W76_RS16880 Protein ID WP_000615979.1
Coordinates 3056570..3056848 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6W76_RS16875 Protein ID WP_000729704.1
Coordinates 3056307..3056567 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W76_RS16850 3052090..3052875 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
C6W76_RS16855 3052847..3054559 + 1713 Protein_3089 flagellar biosynthesis protein FlhA -
C6W76_RS16860 3054664..3054942 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6W76_RS16865 3054935..3055291 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6W76_RS16870 3055348..3056121 - 774 WP_000543899.1 C40 family peptidase -
C6W76_RS16875 3056307..3056567 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6W76_RS16880 3056570..3056848 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6W76_RS16885 3057004..3057744 + 741 WP_001225679.1 murein L,D-transpeptidase -
C6W76_RS16890 3057715..3058482 - 768 WP_001365365.1 class II glutamine amidotransferase -
C6W76_RS16895 3058688..3059266 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T99038 WP_000615979.1 NZ_CP027459:3056570-3056848 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T99038 NZ_CP027459:3056570-3056848 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT99038 WP_000729704.1 NZ_CP027459:3056307-3056567 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT99038 NZ_CP027459:3056307-3056567 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References