Detailed information of TA system TA099022

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 78744..78997
• Replicon: plasmid unnamed
• Accession: NZ_CP027458
• Organism: Escherichia coli strain 88-3493

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: C6W79_RS26065
• Protein ID: WP_001336447.1
• Coordinates: 78744..78893 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	78941..78997 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W79_RS26020	74278..74700	-	423	WP_028985400.1	Exc2 family lipoprotein	-
C6W79_RS26025	74983..75498	+	516	Protein_89	transposase	-
C6W79_RS26030	75829..76068	-	240	WP_000859018.1	MarR family transcriptional regulator	-
C6W79_RS26045	77044..77901	-	858	WP_000130991.1	incFII family plasmid replication initiator RepA	-
C6W79_RS26050	77894..77968	-	75	WP_001365705.1	RepA leader peptide Tap	-
C6W79_RS26060	78203..78460	-	258	WP_000084404.1	replication regulatory protein RepA	-
C6W79_RS26065	78744..78893	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	78941..78997	+	57	NuclAT_1	-	Antitoxin
-	78941..78997	+	57	NuclAT_1	-	Antitoxin
-	78941..78997	+	57	NuclAT_1	-	Antitoxin
-	78941..78997	+	57	NuclAT_1	-	Antitoxin
C6W79_RS26080	79657..80118	-	462	WP_000760084.1	thermonuclease family protein	-
C6W79_RS26090	81214..81771	-	558	WP_024213676.1	fertility inhibition protein FinO	-
C6W79_RS26095	81807..82175	-	369	WP_000722534.1	NirD/YgiW/YdeI family stress tolerance protein	-
C6W79_RS26100	82258..83004	-	747	WP_024213675.1	type-F conjugative transfer system pilin acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC	1..107796	107796
	-	flank	IS/Tn	-	-	75096..75530	434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T99022 WP_001336447.1 NZ_CP027458:c78893-78744 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T99022 NZ_CP027458:c78893-78744 [Escherichia coli]
ATGACGAAATATACCCTTATTGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT99022 NZ_CP027458:78941-78997 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin