Detailed information of TA system TA099017

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13437..13854
• Replicon: plasmid unnamed
• Accession: NZ_CP027458
• Organism: Escherichia coli strain 88-3493

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: C6W79_RS25620
• Protein ID: WP_000775238.1
• Coordinates: 13437..13598 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	13663..13854 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W79_RS25575	9249..9632	-	384	WP_001585112.1	relaxosome protein TraM	-
C6W79_RS25580	9953..10555	+	603	WP_106888575.1	transglycosylase SLT domain-containing protein	-
C6W79_RS25585	10851..11672	-	822	WP_024213651.1	DUF945 domain-containing protein	-
C6W79_RS25590	11791..12078	-	288	WP_000107542.1	hypothetical protein	-
C6W79_RS25600	12234..12536	-	303	WP_024213649.1	hypothetical protein	-
C6W79_RS26620	12839..13136	+	298	Protein_21	hypothetical protein	-
C6W79_RS25620	13437..13598	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	13663..13854	+	192	-	-	Antitoxin
-	13663..13857	-	195	NuclAT_0	-	-
-	13663..13857	-	195	NuclAT_0	-	-
-	13663..13857	-	195	NuclAT_0	-	-
-	13663..13857	-	195	NuclAT_0	-	-
C6W79_RS25625	13806..14120	-	315	Protein_23	theronine dehydrogenase	-
C6W79_RS25630	14117..14836	-	720	WP_001276237.1	plasmid SOS inhibition protein A	-
C6W79_RS25635	14833..15267	-	435	WP_024213646.1	conjugation system SOS inhibitor PsiB	-
C6W79_RS25640	15322..17280	-	1959	WP_052892458.1	ParB/RepB/Spo0J family partition protein	-
C6W79_RS25645	17346..17579	-	234	WP_021564200.1	DUF905 family protein	-
C6W79_RS25650	17637..18170	-	534	WP_024213643.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC	1..107796	107796

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T99017 WP_000775238.1 NZ_CP027458:c13598-13437 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T99017 NZ_CP027458:c13598-13437 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 192 bp

>AT99017 NZ_CP027458:13663-13854 [Escherichia coli]
GCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACAACACGGTAACAAACCACCTTCACGTC
ATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCAGCACGCTGACCACACTCACTTTCCCTGAAAAT
AATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin