Detailed information of TA system TA098988

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20892..21318
• Replicon: plasmid unnamed1
• Accession: NZ_CP027455
• Organism: Escherichia coli strain 2014C-4423

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C6W74_RS28570
• Protein ID: WP_001312861.1
• Coordinates: 20892..21050 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	21094..21318 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W74_RS28530	16770..17444	+	675	WP_106901533.1	IS66-like element accessory protein TnpA	-
C6W74_RS28535	17441..17788	+	348	WP_000631725.1	IS66 family insertion sequence element accessory protein TnpB	-
C6W74_RS28540	17808..19364	+	1557	WP_001066419.1	IS66 family transposase	-
C6W74_RS28545	19393..19620	-	228	WP_106901580.1	hypothetical protein	-
C6W74_RS28550	19679..20002	-	324	WP_000533253.1	hypothetical protein	-
C6W74_RS30055	20298..20657	+	360	WP_024223173.1	hypothetical protein	-
C6W74_RS28570	20892..21050	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	21094..21318	-	225	NuclAT_0	-	Antitoxin
-	21094..21318	-	225	NuclAT_0	-	Antitoxin
-	21094..21318	-	225	NuclAT_0	-	Antitoxin
-	21094..21318	-	225	NuclAT_0	-	Antitoxin
C6W74_RS28580	21330..22049	-	720	WP_021553187.1	plasmid SOS inhibition protein A	-
C6W74_RS28585	22046..22480	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C6W74_RS28590	22549..24514	-	1966	Protein_34	ParB/RepB/Spo0J family partition protein	-
C6W74_RS28595	24578..24811	-	234	WP_021553185.1	DUF905 domain-containing protein	-
C6W74_RS28605	24869..25390	-	522	WP_021553184.1	single-stranded DNA-binding protein	-
C6W74_RS30060	25735..25959	+	225	WP_013279293.1	hypothetical protein	-
C6W74_RS28630	26033..26257	-	225	Protein_38	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..73262	73262

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T98988 WP_001312861.1 NZ_CP027455:c21050-20892 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T98988 NZ_CP027455:c21050-20892 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT98988 NZ_CP027455:c21318-21094 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGACTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCATGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin