Detailed information of TA system TA098980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4737765..4738492
• Replicon: chromosome
• Accession: NZ_CP027454
• Organism: Escherichia coli strain 2014C-4423

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: C6W74_RS25150
• Protein ID: WP_000547564.1
• Coordinates: 4738181..4738492 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6W74_RS25145
• Protein ID: WP_000126294.1
• Coordinates: 4737765..4738184 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W74_RS25125	4732895..4733422	-	528	WP_001078777.1	electron transport protein HydN	-
C6W74_RS25130	4733571..4734581	-	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
C6W74_RS25135	4734841..4736217	+	1377	WP_001107877.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C6W74_RS25140	4736226..4737650	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
C6W74_RS25145	4737765..4738184	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C6W74_RS25150	4738181..4738492	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6W74_RS25155	4738666..4739427	-	762	WP_001026445.1	hypothetical protein	-
C6W74_RS25160	4739452..4739922	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C6W74_RS25165	4739915..4740325	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C6W74_RS25170	4740322..4741089	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
C6W74_RS25175	4741089..4741631	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
C6W74_RS25180	4741641..4743350	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T98980 WP_000547564.1 NZ_CP027454:c4738492-4738181 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T98980 NZ_CP027454:c4738492-4738181 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT98980 WP_000126294.1 NZ_CP027454:c4738184-4737765 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT98980 NZ_CP027454:c4738184-4737765 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5