Detailed information of TA system TA098965

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3091536..3091907
• Replicon: chromosome
• Accession: NZ_CP027454
• Organism: Escherichia coli strain 2014C-4423

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: C6W74_RS16430
• Protein ID: WP_001317028.1
• Coordinates: 3091713..3091907 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3091536..3091714 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W74_RS16400	3087288..3087461	+	174	WP_001296046.1	hypothetical protein	-
C6W74_RS16405	3087491..3088864	+	1374	WP_000123758.1	ATP-dependent RNA helicase DbpA	-
C6W74_RS16410	3088993..3089928	-	936	WP_001157421.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C6W74_RS16415	3089980..3091215	-	1236	WP_000040852.1	site-specific integrase	-
C6W74_RS16420	3091217..3091432	-	216	WP_000079604.1	excisionase XisR	-
C6W74_RS16425	3091511..3091720	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	3091536..3091714	+	179	NuclAT_0	-	Antitoxin
-	3091536..3091714	+	179	NuclAT_0	-	Antitoxin
-	3091536..3091714	+	179	NuclAT_0	-	Antitoxin
-	3091536..3091714	+	179	NuclAT_0	-	Antitoxin
C6W74_RS16430	3091713..3091907	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C6W74_RS16435	3091964..3092773	-	810	WP_000595429.1	recombination protein RecT	-
C6W74_RS16440	3092766..3095435	-	2670	WP_000102191.1	exodeoxyribonuclease VIII	-
C6W74_RS16445	3095516..3095692	-	177	WP_000245534.1	hypothetical protein	-
C6W74_RS16450	3095686..3095907	-	222	WP_000560223.1	killing protein KilR	-
C6W74_RS16455	3096332..3096484	-	153	WP_050437129.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	3089980..3138704	48724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T98965 WP_001317028.1 NZ_CP027454:c3091907-3091713 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T98965 NZ_CP027454:c3091907-3091713 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT98965 NZ_CP027454:3091536-3091714 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin